پژوهشگاه ملی مهندسی ژنتیک

In the name of God

The University of Guilan

2001-2006: Ph.D student, Clinical Biochemistry, Tarbiat Modarres University, Tehran, Iran.

1996-2000: Lecturer, Department of Biology, Faculty of Sciences, Guilan University, Rasht, Iran

1993-1996: Received M.Sc. in Medical Biochemistry, Tehran University of Medical Sciences, Tehran, Iran

1989-1993: Received B.Sc. in Biology, Faculty of Sciences, Ferdoosi University, Mashad, Iran

I have experience in IEF, RNA extraction, DNA purification, cloning,

expression, and purification of protein using prokaryotic and eukaryotic yeast, Pichia

pastoris), mammalian cell culture, assay and kinetic of enzyme.

I have experience in advising the B.Sc. , M.Sc. , Ph.D. and M.D. Student projects

Computer Skills: Various biological soft wares (Gene Runner, Gel Imaging Software, RNA draw, Blast, Oligo …), Windows program include Word, Excel, Photoshop, PowerPoint and…)

M.Sc. Project: Assessment of PGI and ALD Activity and its Isoenzymes Pattern in Mucosal Tissues of Esophagus in Cancerous (SCC) and Healthy Subjects.

Ph.D. Project: Assessment of Yeast Intron Insertion on mRNA Level and Expression Rate of

1- Oxidative stress level and dermal Tyrosinase activity in Iranian Vitiligo Patients. Eskandani M., Golchai J., Pirooznia N., Hasannia S., Indian journal of dermatology, Vol. 1(55),  9-15, 2010.

2- Assessment of antifungal effects of Pterocariya fraxinifolia for Cream formulation. Submitted in Evidence based complementary and alternative medicine (EBCAM), USA, 2009

Unacylated Ghrelin levels increase after aerobic exercise program in obese women. Mirzaei, B., Iran Doost, Kh., Rahmani Nia, F., Mohebi, H., Hasannia, S. Brazilian Journal of Biochemistry (BRJB),

Vol. 3(1

3- Association among lifestyle status, plasma adiponectin level and metabolic syndrome in obese middle aged men. Mohebi, Moghadasi M., H., Rahmani Nia, F., Hasannia, S., Noroozi H. Brazilian Journal of Biochemistry (BRJB), Vol. 3(3), 243-252, 2009.

4-  Sequence and structural analysis of artemin based on ferritin: A comparative study, Behnam Rasti , S. Shirin Shahangian, Reza H. Sajedi , Majid Taghdir, Sadegh Hasannia a, Bijan Ranjbar,

Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics

5- Anti-MUC1 Nanobody Can Redirect T-Body Cytotoxic Effector Function, Seyed Hamid Aghaee Bakhtiari,1 Fatemeh Rahbarizadeh,1 Sadegh Hasannia,2, Davoud Ahmadvand,3 Farnoush Jafari Iri-Sofla,1 and Mohammad Javad Rasaee1, HYBRIDOMA, Vol. 28, No.2

, 85-92 , 2009, Mary Ann Liebert, Inc.

6- Unacylated Ghrelin levels increase after aerobic exercise program in obese women. Mirzaei, B., Iran Doost, Kh., Rahmani Nia, F., Mohebi, H., Hasannia, S. Brazilian Journal of Biochemistry (BRJB), 2009/Februrary 1(3) , 11-20

7-The relationship between cholestryl ester transfer protein Taq I genotype and plasma level of HDL subgroups. in coronary heart disease. S. Hasannia, S. Jalili and R. Sariri*. Biosciences, Biotechnology Research Asia, Vol. 5(1), 447-450, 2008

8- Assessment of MC1R and α-MSH genes sequences in Iranian Vitiligo Patients. Hasannia S., Eskandani M., pirooznia N., and Golchai, Indian Journal of Dermatology, 2008.

9- Rapid alpha-1-antitrypsin M-variant genotyping by primer-induced restriction analysis. Yajloo MM, Lotfi AS, Mesbah Namin SA, Hasannia S, Biglarzadeh M, Ardalan A.

Diagn Mol Pathol. 2007 Mar;16(1):54-6.

10- A study of the oxidation-induced conformational and functional changes in neuroserpin. Mohsenifar Afshin, Lotfi Abbas S, Ranjbar Bijan, Allameh Abdolamir, Zaker Farhad, Hasani Leila, Kia Batool Etemadi, Hasannia Sadegh, Iranian biomedical journal, Vol. 11(1),41-46, 2007.

11- Oxidation of Neuroserpin, a Conformational Disorder; Study of Fluorescence Intensity, and Inhibitory Activity. Afshin Mohsenifar, Abbas S. Lotfi1*, Bijan Ranjbar, Abdolamir Allameh1, Batool Etemadi Kia, Sadegh Hasannia, IJCB, 2006. In Press

12-Elevated Expression of Human Alpha-1 Antitrypsin Mediated by Yeast Intron in Pichia pastoris. S. Hasannia¹, A.S. Lotfi ^1*, F. Mahboudi², A. Rezaii¹, F. Rahbarizadeh³ and A. Mohsenifar^1.. Biotechnology letters. 2006 , 28:1545-1550

13- مقایسه میزان اکسیداسیون چربی در دامنه شدت های فعالیت دویدن دانشجویان پسر غیر ورزشکار، هادی روحانی ،ارسلان دمیرچی، صادق حسن نیا ، زهرا روحانی، المپیک، (45)17، 121-130. 1388.

14- بررسی اثر کافئین بر فشار خون در هنگام در هنگام فعالیت زیر بیشینه و استراحت در افراد دارای اضافه وزن، ارسلان دمیرچی، فرهاد رحمانی نیا، بهمن میرزایی، صادق حسن نیا، و محسن ابراهیمی،

غدد درون ریز و متابولیسم ایران، (6)10،628-623، 1387

15- تاثیر کم آبی بر حداکثر اکسیداسیون چربی (MFO) و شدت فعالیت متناسب با MFO(Fatmax)، هادی روحانی، ارسلان دمیرچی، صادق حسن نیا و میثم روحانی،

المپیک، (44)16 ،98 -89، 1387

16- اثر تمرین هوازی بر غلظت لپتین پلاسمایی زنان با وزن طبیعی و ارتباط آن با چربی زیر پوستی ناحیه شکم، خدیجه ایراندوست، فرهاد رحمانی نیا، حمید محبی، بهمن میرزایی، و صادق حسن نیا، علوم حرکت انسان، (2) 16، 160-151،1387

17- اثر چاقی بر اکسیداسیون چربی و کربوهیدرات در طی فعالیت زیر بیشینه،فرهاد رحمانی نیا ، ارسلان دمیرچی،بهمن میرزایی،صادق حسن نیا، محسن ابراهیمی،

پژوهش در علوم ورزشی، 1387

18- اثر تمرین هوازی بر مولکول های آسیل دار و غیر آسیل دار گرلین در زنان چاق؛خدیجه ایراندوست، فرهاد رحمانی نیا، حمید محبی، بهمن میرزایی، و صادق حسن نیا، پژوهش در علوم ورزشی، 1387

19- اثر تمرین هوازی بر غلظت گرلین و لپتین پلاسمایی زنان چاق و با وزن طبیعی، خدیجه ایراندوست، فرهاد رحمانی نیا، حمید محبی، بهمن میرزایی، و صادق حسن نیا، المپیک، (2)18 ،99 -87، 1389

20- اثر دوازده هفته فعالیت های زندگی بر بیان ژن و غلظت پلاسمایی آدیپونکتین در مردان چاق، حمید محبی، مهرزاد مقدسی، فرهاد رحمانی نیا، صادق حسن نیا، و حمید نوروزی، ، غدد درون ریز و متابولیسم ایران، (1) 12- 48-40، 1389

2- Creation (Molecular Evolution) ……………………..…………………in Farsi

4- Metabolism Regulation in Mamals……………………………………….in Farsi (in press)

1--PURIFICATION OF PROTEIN CONTAINS SIX HISTIDINE TAG USING A METAL CHELATING RESIN, NI-SP50. Mohsenifar A.1, Lotfi A.S.1, Ranjbar B.2, Allameh A.1, Hasannia S.3,Rahbarizadeh F.1, Omidfar K.1, Nikkhah M.4, Khoshdel A.1, Etemadi Kia B.1, 8th ICB and first

ICBMB, TMU, Tehran, Iran , 2005

2-ALPHA-1 ANTITRYPSIN (AAT) ACTIVITY , PHENOTYPES AND ITS CORRELATION WITH ANCA IN IRANIAN PATIENTS WITH AUTIIMMUNE DISEASES.Lotfi A.S.1,Khoshdel A.1, Mansori p.2, Sarafnejad T.3,Mohsenifar A.1, Etemadi Kia B.1, , and Hasannia S.4, 8th ICB and first ICBMB, TMU, Tehran, Iran , 2005

Sarafnejad A.3 Shahrestani T.3,Mohsenifar A.1, Etemadi Kia B.1, Afshar Naderi A1.,and Hasannia S.4, 8th ICB and first ICBMB, TMU, Tehran, Iran , 2005

4-Effect of a yeast homologous intron on expression rate of Heterologous protein from Pichia pastoris.

Hasannia S. Lotfi A.S, Rezaii A., Mahboodi F., Etemadi-kia B and Mohsenifar A. 8th ICB and first

ICBMB, TMU, Tehran, Iran , 2005

5- Interaction between IgA & Acute Phase Protein Serine Proteinase Inhibitor In Vitro.Lotfi A.S., Moosavi A., Farzami B., Mohsenifar A., Hasannia S., Goodarzi B.G.The 7^th Iranian Congress of Biochemistry, Ahvaz, Iran, Jan 26-29, 2004

6- Basic Structure of Alpha1-Antitrypsin as a Suitable Pattern in study of Conformational Disease.Lotfi A.S., Zahiri H.R., Emami H.R., Hasannia S., Mohsenifar A., The 7th Iranian Congress of Biochemistry, Ahvaz, Iran, Jan 26-29, 2004

7- Some Biochemical Aspects in Patients with Gallstone Disease. Zahiri H.R., Lotfi A.S., Emami H.R , Movahedi M., Hasannia S. The 7th Iranian Congress of Biochemistry, Ahvaz, Iran, Jan 26-29, 2004

8- New Pattern of PGI Isoenzyme as A Diagnostic Marker of Esophageal Squamous Cell

Carcinoma. Hasannia S.1, Lotfi A.S.2, Rooshan M.K.3, Khoshdel A.2, Mohsenifar A.4. .22nd

International Congress of Pathology & Laboratory Medicine, Busan, South Korea, Aug 30 -

9-New Application of HDL2-C in Assessment of Patients with Coronary Heart Disease

Mohsenifar A. Hasannia S., Mir Blook F., Shaad B., Samami N.22nd International Congress of Pathology & Laboratory Medicine, Busan, South Korea, Aug 30 -September 3, 2003, (Young Investigator Award)

10-The Relationship between Plasma Level of lipoproteins a, Lipid Status and Coronary Heart

Disease in Patients Underwent Coronary Angiography. Mirblook F., Hasannia S., Shaad B. and

11- HDL-2 Is More Important Anti-Risk Factor in Coronary Heart Disease , Journal of Medical Sciences, Rafsanjan , 4 Mahmoodi . Mohsenifar, M . Mir Blook, A . , F Hasannia . S. University of medical Sciences, Vol2, No.2 . 2003

12-Increased Excretion of Fatty Acid Metabolites in Urine of Patients with Chain Acyl-CoA

Dehydrogenase as an Indicator in Diagnosis of this Disease. Mohsenifar A., Lotfi A.S., Zaman, T.

Kazemnejad A. and S. Hasannia, The 18th International Congress of Clinical Chemistry and Laboratory Medicine, 20-25 Oct. 2002, Kyoto, Japan. ------------------------------ (AWARD)

13- Aldolase (EC=4.1.2.13) in Esophageal Cancer (SCC) and Healthy Subjects.

I.R.Iran. 31 Aug. – 2 Sep. 1999

14- Assessment of PGI activity and its isoenzyme patterns in squamous cell carcinoma,

Sciences, Tehran University of medical Sciences, 25-29 Sep. 1997

15- Construction of a new functional α-1 antitrypsin with reduced size, Sadegh Hasannia, Nazanin Pirooznia, E. Dasi Sangachini, M. Taghdir, K. Khalili Ghadikolai, The thenth Iranian congress of biochemistry and 3^rd international congress of biochemistry and molecular biology, Tehran, Iran, November 16-19, 2009.

 Projects (supervisor and advisor) :

Relation between Plasma Level of HDL2-C & HDL3-C in Patients with Coronary HeartDisease

Angiography.Mir Blook F. Hasannia S Sponsored by: Guilan Medical Sciences University

The Construction of Chimeric T-cell Receptor with Spacer base of Modeling Study of VHH and MUC1 Interaction ( Nazanin Pirooznia, as Msc project)

Cloning, Sequencing and Characterization of Artemin from Artemia urmiana by Experimental and Theoretical Methods (Behnam Rasti, as Msc project)

Assessment of Expression, Isolation and Biochemical Characterization of Taq DNA Polymerase in Prokaryotic Host (Leila Azizzade Pormehr, as Msc project)

Assessment of MC1R Gene Sequence and Oxidative Stress in Vitiligo Patient (Morteza Eskandani, as MSc project)

Clinical Biochemistry: For Students Medicine and Laboratory Sciences, Rafsangan Medical

Sciences University (1997), Biochemistry: For Students of Biology and Agricultural Sciences, Guilan University, Rasht, Iran, (1998-Now). Metabolic Regulation: For student of Biochemistry (M.Sc), Guilan University, Rasht, Iran (2003). Radiobiology: For Students of Biology, Guilan University, Rasht, Iran (1998-2002). Biochemistry of Cell Membrane, For student of Biochemistry (M.Sc), Guilan University, Rasht, Iran (2004-now). Immunobiology: For student of Biochemistry (M.Sc), Guilan University, Rasht, Iran (2006-now)

Workshop: Training and Workshop on Update and Advanced Clinical and general Biochemistry Methods,Tarbiat Modarres University (September 2002)

1-Gene Expression in Eukaryotic Cells, Molecular Analysis of Gene Products. 26-31 July 1997,National Research Center for Genetic Engineering & Biotechnology, Tehran, Iran

2- Real Time PCR (Corbett) Oct. 29 2007, Shiraz Medical University. Shiraz-Iran.